9,966 research outputs found
Well-defined silica-supported olefin metathesis catalysts
Two triethoxysilyl-functionalized N-heterocyclic carbene ligands have been synthesized and used to prepare the corresponding second-generation ruthenium olefin metathesis catalysts. These complexes were then grafted onto silica gel, and the resulting materials were efficient heterogeneous catalysts for a number of metathesis reactions. The solid-supported catalysts were shown to be recyclable over a number of reaction cycles, and no detectable levels of ruthenium were observed in reaction filtrates (ruthenium concentration of filtrate <5 ppb)
Pre-processing for approximate Bayesian computation in image analysis
Most of the existing algorithms for approximate Bayesian computation (ABC)
assume that it is feasible to simulate pseudo-data from the model at each
iteration. However, the computational cost of these simulations can be
prohibitive for high dimensional data. An important example is the Potts model,
which is commonly used in image analysis. Images encountered in real world
applications can have millions of pixels, therefore scalability is a major
concern. We apply ABC with a synthetic likelihood to the hidden Potts model
with additive Gaussian noise. Using a pre-processing step, we fit a binding
function to model the relationship between the model parameters and the
synthetic likelihood parameters. Our numerical experiments demonstrate that the
precomputed binding function dramatically improves the scalability of ABC,
reducing the average runtime required for model fitting from 71 hours to only 7
minutes. We also illustrate the method by estimating the smoothing parameter
for remotely sensed satellite imagery. Without precomputation, Bayesian
inference is impractical for datasets of that scale.Comment: 5th IMS-ISBA joint meeting (MCMSki IV
How to Remove a Dumbbell Tumour of the Sciatic Notch
Purpose.To look at a method for treating soft tissue sarcoma of the
retroperitoneal area
Cyclopropylamine inactivation of cytochromes P450: Role of metabolic intermediate complexes
NOTICE: this is the author’s version of a work that was accepted for publication in Archives of Biochemistry and Biophysics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Archives of Biochemistry and Biophysics, Volume 436, Issue 2, 15 April 2005 doi:10.1016/j.abb.2005.02.020The inactivation of cytochrome P450 enzymes by cyclopropylamines has been attributed to a mechanism involving initial one-electron oxidation at nitrogen followed by scission of the cyclopropane ring leading to covalent modification of the enzyme. Herein, we report that in liver microsomes N-cyclopropylbenzylamine (1) and related compounds inactivate P450 to a large extent via formation of metabolic intermediate complexes (MICs) in which a nitroso metabolite coordinates tightly to the heme iron, thereby preventing turnover. MIC formation from 1 does not occur in reconstituted P450 systems with CYP2B1/2, 2C11 or 2E1, or in microsomes exposed to gentle heating to inactivate the flavin-containing monooxygenase (FMO). In contrast, N-hydroxy-N-cyclopropylbenzylamine (3) and N-benzylhydroxylamine (4) generate MICs much faster than 1 in both reconstituted and microsomal systems. MIC formation from nitrone 5 (PhCH = N(O)cPr) is somewhat faster than from 1, but very much faster than the hydrolysis of 5 to a primary hydroxylamine. Thus the major overall route from 1 to a P450 MIC complex would appear to involve FMO oxidation to 3, further oxidation by P450 and/or FMO to nitrone 5′ (C2H4C = N(O)CH2Ph), hydrolysis to 4, and P450 oxidation to α-nitrosotoluene as the precursor to oxime 2 and the major MIC from 1
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